Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.Īpproximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 ☌), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). The ladder is supplied in gel loading buffer and is ready to use. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). WesternSure ® Pre-stained Protein Ladder. Odyssey ® One-Color Protein Molecular Weight Marker. The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Chameleon ® NIR Prestained Protein Ladders. 1.5-2.5 μL per well for general Western transfering.īLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane. Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Custom Recombinant Antibody Services - antibodies-online The ScanLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins as well as three prestained marker proteins. This protein marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on.RNA Based Therapeutics - Drug Discovery.Stronger blot persistence provides enhanced: Confidence after western blot processing. Increased brightness makes it easier to: Identify target proteins on the gel or blot. Annexin V-FITC Apoptosis Detection Kits Improved Dual Color Standards are brighter than the competition on both gels and blots.If you have any questions or need any help, please contact us. To place your order, you will need the Abcam product code equivalent for your BioVision product. You can find this by entering your BioVision product code into the search bar at the top of the page.įor information on placing an order, including payment options click here. If you are new to Abcam, you can easily find all the information you need to help you purchase from us here, including how to set up your account and how to search and find products. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. If you are unable to find your product, then please contact us. You can be confident that this is the same product because the product overview section of our datasheet will reference the BioVision catalog number you searched.įor example, if you enter K303 into the main Abcam search bar, it will return Cell Viability assay kit (fluorometric- blue) ab102501, which is the same product as ‘VisionBlue ™ Quick Cell Viability Fluorometic Assay K303’ at BioVision. proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.Molecular-weight size markers are a set of. To find your product, simply enter your BioVision catalog number in the search bar at the top of the page. The resulting search will show the equivalent Abcam product name and catalog number. We’ve taken steps to ensure you can easily find your BioVision products on our catalog for a seamless transition and minimal interruption to your research. Flexible delivery solutions we ship to 143 countries worldwide by express courier delivery for the fastest transit times.Unparalleled support we have multi-lingual scientific support and customer service teams that aim to respond to your inquiry within 24 hours, if not sooner.
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